DanskIndexContactPhone bookDTU AlumniPortalenUniversity LibraryCareer
Phone book
Title: Drug quantification in turbid media by fluorescence imaging combined with light-absorption correction using white Monte Carlo simulations
Type: Journal articleJournal article
Participant(s):
Forfatter:  Xie, Haiyan
Lund University, Department of Physics

Forfatter:  Liu, Haichun
Lund University, Department of Physics

Forfatter:  Svenmarker, Pontus
Lund University, Department of Physics

Forfatter:  Axelsson, Johan
Lund University, Department of Physics

Forfatter:  Xu, Can T.
Lund University, Department of Physics

Forfatter:  Gräfe, Susanna
Biolitec AG

Author:  Lundeman, Jesper Holm (Cwisno: 14029)
Technical University of Denmark
Email:

Author:  Cheng, Haynes Pak Hay (Cwisno: 44911)
Technical University of Denmark
Email:

Forfatter:  Svanberg, Sune
Lund University, Department of Physics

Forfatter:  Bendsoe, Niels
Lund University Hospital, Department of Dermatology and Venereology

Author:  Andersen, Peter E. (Cwisno: 7659)
Technical University of Denmark
Email:

Forfatter:  Svanberg, Katarina
Lund University Hospital, Department of Oncology

Forfatter:  Andersson-Engels, Stefan
Lund University, Department of Physics
Abstract: Accurate quantification of photosensitizers is in many cases a critical issue in photodynamic therapy. As a noninvasive and sensitive tool, fluorescence imaging has attracted particular interest for quantification in pre-clinical research. However, due to the absorption of excitation and emission light by turbid media, such as biological tissue, the detected fluorescence signal does not have a simple and unique dependence on the fluorophore concentration for different tissues, but depends in a complex way on other parameters as well. For this reason, little has been done on drug quantification in vivo by the fluorescence imaging technique. In this paper we present a novel approach to compensate for the light absorption in homogeneous turbid media both for the excitation and emission light, utilizing time-resolved fluorescence white Monte Carlo simulations combined with the Beer-Lambert law. This method shows that the corrected fluorescence intensity is almost proportional to the absolute fluorophore concentration. The results on controllable tissue phantoms and murine tissues are presented and show good correlations between the evaluated fluorescence intensities after the light-absorption correction and absolute fluorophore concentrations. These results suggest that the technique potentially provides the means to quantify the fluorophore concentration from fluorescence images. © 2011 Society of Photo-Optical Instrumentation Engineers (SPIE).
Published: in journal: Journal of Biomedical Optics (ISSN: 1083-3668) (DOI: http://dx.doi.org/10.1117/1.3585675), vol: 16, issue: 6, pages: 066002, 2011
DOI:
See the publication in DTU Orbit See the publication in DTU Orbit

Top
Anker Engelundsvej 1Building 101A2800 Kgs. LyngbyTel +45 45 25 25 25VAT 30 06 09 46EAN
CookiesDTU på YouTubeDTU på Facebook