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| Title:
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Copper amendment of agricultural soil selects for bacterial antibiotic resistance in the field |
| Type:
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Journal articleJournal article |
| Participant(s):
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Forfatter:
Berg, J.
Technical University of Denmark, National Veterinary Institute
Forfatter:
Tom-Petersen, A.
Technical University of Denmark, National Veterinary Institute
Forfatter:
Nybroe, O.
Technical University of Denmark
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| Abstract:
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Aims: The objective of this study was to determine whether Cu-amendment of field plots affects the frequency of Cu resistance, and antibiotic resistance patterns in indigenous soil bacteria. Methods and Results: Soil bacteria were isolated from untreated and Cu-amended field plots. Cu-amendment significantly increased the frequency of Cu-resistant isolates. A panel of isolates were characterized by Gram-reaction, amplified ribosomal DNA restriction analysis and resistance profiling against seven antibiotics. More than 95% of the Cu-resistant isolates were Gram-negative. Cu-resistant Gram-negative isolates had significantly higher incidence of resistance to ampicillin, sulphanilamide and multiple (greater than or equal to3) antibiotics than Cu-sensitive Gram-negative isolates. Furthermore, Cu-resistant Gram-negative isolates from Cu-contaminated plots had significantly higher incidence of resistance to chloramphenicol and multiple (greater than or equal to2) antibiotics than corresponding isolates from control plots. Significance and Impact of the Study: The results of this field experiment show that introduction of Cu to agricultural soil selects for Cu resistance, but also indirectly selects for antibiotic resistance in the Cu-resistant bacteria. Hence, the widespread accumulation of Cu in agricultural soils worldwide could have a significant effect on the environmental selection of antibiotic resistance. |
| Published:
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in journal: Letters in Applied Microbiology (ISSN: 0266-8254) (DOI: http://dx.doi.org/10.1111/j.1472-765X.2004.01650.x), vol: 40, issue: 2, pages: 146-151, 2005 |
| DOI:
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