In vitro growth of four individual human gut bacteria on oligosaccharides produced by chemoenzymatic synthesis.

Vigsnæs, Louise Kristine ; Nakai, Hiroyuki ; Hemmingsen, Lene ; Andersen, Joakim Mark ; Lahtinen, Sampo J ; Rasmussen, Louise Enggaard ; Abou Hachem, Maher ; Petersen, Bent O. ; Duus, Jens Øllgaard ; Meyer, Anne S. ; Licht, Tine Rask ; Svensson, Birte

in journal: Food & Function (ISSN: 2042-6496) (DOI: http://dx.doi.org/10.1039/C3FO30357H), vol: 4, issue: 5, pages: 784-793, 2013

Type: Journal article - Journal article

DOI: 10.1039/C3FO30357H

Raffinose family oligosaccharide utilisation by probiotic bacteria: insight into substrate recognition, molecular architecture and diversity of GH36 alpha-galactosidases

Abou Hachem, Maher ; Fredslund, Folmer ; Andersen, Joakim Mark ; Larsen, Rene Jonsgaard ; Majumder, Avishek ; Hansen, Morten Ejby ; van Zanten, Gabriella Christina ; Lahtinen, S. J. ; Barrangou, R. ; Klaenhammer, T. ; Jacobsen, S. ; Coutinho, P. M. ; Lo Leggio, Leila ; Svensson, Birte

in journal: Biocatalysis and Biotransformation (ISSN: 1024-2422) (DOI: http://dx.doi.org/10.3109/10242422.2012.674717), vol: 30, issue: 3, pages: 316-325, 2012

Type: Journal article - Journal article

DOI: 10.3109/10242422.2012.674717

Enzymology and Structure of the GH13_31 Glucan 1,6-α-Glucosidase That Confers Isomaltooligosaccharide Utilization in the Probiotic Lactobacillus acidophilus NCFM

Møller, Marie Sofie ; Fredslund, Folmer ; Majumder, Avishek ; Nakai, Hiroyuki ; Poulsen, Jens-Christian Navarro ; Lo Leggio, Leila ; Svensson, Birte ; Abou Hachem, Maher

in journal: Journal of Bacteriology (ISSN: 0021-9193) (DOI: http://dx.doi.org/10.1128/JB.00622-12), vol: 194, issue: 16, pages: 4249-4259, 2012

Type: Journal article - Journal article

DOI: 10.1128/JB.00622-12

Structure of the starch-debranching enzyme barley limit dextrinase reveals homology of the N-terminal domain to CBM21

Møller, Marie Sofie ; Abou Hachem, Maher ; Svensson, Birte ; Henriksen, Anette

in journal: Acta Crystallographica. Section F: Structural Biology and Crystallization Communications Online (ISSN: 1744-3091) (DOI: http://dx.doi.org/10.1107/S1744309112031004), vol: 68, issue: Pt 9, pages: 1008-1012, 2012

Type: Journal article - Journal article

DOI: 10.1107/S1744309112031004

Transcriptional Analysis of Prebiotic Uptake and Catabolism by Lactobacillus acidophilus NCFM.

Andersen, Joakim Mark ; Barrangou, Rodolphe ; Abou Hachem, Maher ; Lahtinen, Sampo J ; Goh, Yong-Jun ; Svensson, Birte ; Klaenhammer, Todd R

in journal: P L o S One (ISSN: 1932-6203), vol: 7, issue: 9, pages: e44409, 2012

Type: Journal article - Journal article

Degradation of the starch components amylopectin and amylose by barley α-amylase 1: Role of surface binding site 2

Nielsen, Jonas Willum ; Kramhøft, Birte ; Bozonnet, Sophie ; Abou Hachem, Maher ; Stipp, Susan Louise Svane ; Svensson, Birte ; Willemoes, Martin

in journal: Archives of Biochemistry and Biophysics (ISSN: 0003-9861) (DOI: http://dx.doi.org/10.1016/j.abb.2012.08.005), vol: 528, issue: 1, pages: 1-6, 2012

Type: Journal article - Journal article

DOI: 10.1016/j.abb.2012.08.005

Isothermal titration calorimetry and surface plasmon resonance allow quantifying substrate binding to different binding sites of Bacillus subtilis xylanase

Cuyvers, Sven ; Dornez, Emmie ; Abou Hachem, Maher ; Svensson, Birte ; Hothorn, Michael ; Chory, Joanne ; Delcour, Jan A. ; Courtin, Christophe M.

in journal: Analytical Biochemistry (ISSN: 0003-2697) (DOI: http://dx.doi.org/10.1016/j.ab.2011.09.005), vol: 420, issue: 1, pages: 90-92, 2012

Type: Journal article - Journal article

DOI: 10.1016/j.ab.2011.09.005

The relationship between probiotics and prebiotic carbohydrates. Chemoenzymatic synthesis and differential proteome analysis

Nakai, Hiroyuki ; Majumder, Avishek ; Dilokpimol, Adiphol ; Hansen, Morten Ejby ; Abou Hachem, Maher ; Lahtinen, S. ; Jacobsen, Susanne ; Svensson, Birte

Type: Conference abstract for conference - Conference abstract for conference

Presented at: 4th Conference on Biocatalysis in the Food and Drink Industries, Toulouse

Spanning ’integrated omics’ and green chemistry to discover prebiotic oligosaccharides

Svensson, Birte ; Majumder, Avishek ; Hansen, Morten Ejby ; Andersen, Joakim Mark ; van Zanten, Gabriella Christina ; Barangou, R. ; Lahtinen, J. ; Klaenhammer, T.R. ; Abou Hachem, Maher ; Jacobsen, Susanne

Type: Conference abstract for conference - Conference abstract for conference

Presented at: LMC Congress Food in Front, Copenhagen

Starch‐binding domains in the CBM45 family – low‐affinity domains from glucan, water dikinase and α‐amylase involved in plastidial starch metabolism

Glaring, Mikkel Andreas ; Baumann, Martin ; Abou Hachem, Maher ; Nakai, Hiroyuki ; Nakai, Natsuko ; Santelia, Diana ; Sigurskjold, Bent W. ; Zeeman, Samuel C. ; Blennow, Andreas ; Svensson, Birte

in journal: F E B S Journal (ISSN: 1742-464X) (DOI: http://dx.doi.org/10.1111/j.1742-4658.2011.08043.x), vol: 278, issue: 7, pages: 1175-1185, 2011

Type: Journal article - Journal article

DOI: 10.1111/j.1742-4658.2011.08043.x

Expression of Enzymatically Inactive Wasp Venom Phospholipase A1 in Pichia pastoris

Borodina, Irina ; Jensen, Bettina M. ; Wagner, Tim ; Abou Hachem, Maher ; Søndergaard, Ib ; Poulsen, Lars Kærgaard

in journal: P L o S One (ISSN: 1932-6203) (DOI: http://dx.doi.org/10.1371/journal.pone.0021267), vol: 6, issue: 6, pages: e21267, 2011

Type: Journal article - Journal article

DOI: 10.1371/journal.pone.0021267

Expression of enzymatically inactive wasp venom phospholipase A1 in Pichia pastoris

Borodina, Irina ; Jensen, Bettina M. ; Wagner, Tim ; Abou Hachem, Maher ; Søndergaard, Ib ; Poulsen, Lars Kærgaard

Type: Conference abstract for conference - Conference abstract for conference

Presented at: 6th Danish Conference on Biotechnology and Molecular Biology, Vejle

Enzymatic synthesis of β-xylosyl-oligosaccharides by transxylosylation using two beta-xylosidases of glycoside hydrolase family 3 from Aspergillus nidulans FGSC A4

Dilokpimol, Adiphol ; Nakai, Hiroyuki ; Gotfredsen, Charlotte Held ; Appeldoorn, Maaike ; Baumann, Martin ; Nakai, Natsuko ; Schols, Henk A. ; Abou Hachem, Maher ; Svensson, Birte

in journal: Carbohydrate Research (ISSN: 0008-6215) (DOI: http://dx.doi.org/10.1016/j.carres.2010.12.010), vol: 346, issue: 3, pages: 421-429, 2011

Type: Journal article - Journal article

DOI: 10.1016/j.carres.2010.12.010

Efficient secretory expression of functional barley limit dextrinase inhibitor by high cell-density fermentation of Pichia pastoris

Jensen, Johanne Mørch ; Vester-Christensen, Malene Bech ; Møller, Marie Sofie ; Bønsager, Birgit Christine ; Christensen, Hans Erik Mølager ; Abou Hachem, Maher ; Svensson, Birte

in journal: Protein Expression and Purification (ISSN: 1046-5928) (DOI: http://dx.doi.org/10.1016/j.pep.2011.04.009), vol: 79, issue: 2, pages: 217-222, 2011

Type: Journal article - Journal article

DOI: 10.1016/j.pep.2011.04.009

Crystal Structure of α-Galactosidase from Lactobacillus acidophilus NCFM: Insight into Tetramer Formation and Substrate Binding

Fredslund, Folmer ; Abou Hachem, Maher ; Larsen, Rene Jonsgaard ; Sørensen, Pernille Gerd ; Coutinho, Pedro M. ; Lo Leggio, Leila ; Svensson, Birte

in journal: Journal of Molecular Biology (ISSN: 0022-2836) (DOI: http://dx.doi.org/10.1016/j.jmb.2011.07.057), vol: 412, issue: 3, pages: 466-480, 2011

Type: Journal article - Journal article

DOI: 10.1016/j.jmb.2011.07.057

Transcriptional and functional analysis of galactooligosaccharide uptake by lacS in Lactobacillus acidophilus

Andersen, Joakim Mark ; Barrangou, Rodolphe ; Abou Hachem, Maher ; Lahtinen, Sampo ; Goh, Yong Jun ; Svensson, Birte ; Klaenhammer, Todd R

in journal: National Academy of Sciences. Proceedings (ISSN: 0027-8424) (DOI: http://dx.doi.org/10.1073/pnas.1114152108), vol: 108, issue: 43, pages: 17785-17790, 2011

Type: Journal article - Journal article

DOI: 10.1073/pnas.1114152108

Recombinant production and characterisation of two related GH5 endo-β-1,4-mannanases from Aspergillus nidulans FGSC A4 showing distinctly different transglycosylation capacity

Dilokpimol, Adiphol ; Nakai, Hiroyuki ; Gotfredsen, Charlotte Held ; Baumann, Martin ; Nakai, Natsuko ; Abou Hachem, Maher ; Svensson, Birte

in journal: B B A - Proteins and Proteomics (ISSN: 1570-9639) (DOI: http://dx.doi.org/10.1016/j.bbapap.2011.08.003), vol: 1814, issue: 12, pages: 1720-1729, 2011

Type: Journal article - Journal article

DOI: 10.1016/j.bbapap.2011.08.003

Secretory expression of functional barley limit dextrinase by Pichia pastoris using high cell-density fermentation

Vester-Christensen, Malene Bech ; Abou Hachem, Maher ; Næsted, Henrik ; Svensson, Birte

in journal: Protein Expression and Purification (ISSN: 1046-5928) (DOI: http://dx.doi.org/10.1016/j.pep.2009.08.016), vol: 69, issue: 1, pages: 112-119, 2010

Type: Journal article - Journal article

DOI: 10.1016/j.pep.2009.08.016

Efficient one-pot enzymatic synthesis of alpha-(1 -> 4)-glucosidic disaccharides through a coupled reaction catalysed by Lactobacillus acidophilus NCFM maltose phosphorylase

Nakai, Hiroyuki ; Dilokpimol, Adiphol ; Abou Hachem, Maher ; Svensson, Birte

in journal: Carbohydrate Research (ISSN: 0008-6215) (DOI: http://dx.doi.org/10.1016/j.carres.2010.03.021), vol: 345, issue: 8, pages: 1061-1064, 2010

Type: Journal article - Journal article

DOI: 10.1016/j.carres.2010.03.021

Genetic Screening Identifies Cyanogenesis-Deficient Mutants of Lotus japonicus and Reveals Enzymatic Specificity in Hydroxynitrile Glucoside Metabolism

Takos, A. ; Lai, D. ; Mikkelsen, L. ; Abou Hachem, Maher ; Shelton, D. ; Motawia, M.S. ; Olsen, C.E. ; Wang, T.L. ; Martin, C. ; Rook, F.

in journal: Plant Cell (ISSN: 1040-4651) (DOI: http://dx.doi.org/10.1105/tpc.109.073502), vol: 22, issue: 5, pages: 1605-1619, 2010

Type: Journal article - Journal article

DOI: 10.1105/tpc.109.073502