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| Titel:
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DNA microarrays immobilized on unmodified plastics in a microfluidic biochip for rapid typing of Avian Influenza Virus |
| Type:
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Article in proceedingsArticle in proceedings |
| Person(er):
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Technical University of Denmark
Email:
Technical University of Denmark
Email:
Technical University of Denmark
Email:
Technical University of Denmark
Technical University of Denmark
Email:
Technical University of Denmark
Email:
Technical University of Denmark
Email:
Technical University of Denmark
Email:
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| Uddrag:
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Polymers are widely used for microfluidic systems,
but fabrication of microarrays on such materials often
requires complicated chemical surface modifications,
which hinders the integration of microarrays into
microfluidic systems. In this paper, we demonstrate that
UV irradiation can be used to directly immobilize
poly(T)poly(C)-tagged DNA oligonucleotide probes on
non-modified plastic surfaces. This one-step,
cost-effective process provides very high immobilization
and hybridization efficiencies and is applicable to many
different types of polymers. Using this microarray
fabrication technique, a portable cyclic olefin copolymer
(COC) microarray device containing eight individually
addressable microfluidic channels was developed for fast
identification of Avian Influenza Virus (AIV) by DNA
hybridization. This plastic biochip offers benefits of low
fabrication cost and parallel processing of multiple
samples, and could be used as a point-of-care device for
clinical diagnostics and gene expression analysis. |
| Publiceret:
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part of: Transducers’11, Beijing, China, June 5-9, 2011 (ISBN: 978-1-4577-0157-3), pages: 878-881, 2011, IEEE, |
| DOI:
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| Præsenteret ved:
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16th International Solid-State Sensors, Actuators and Microsystems Conference, Beijing |
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