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| Titel:
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Genetic diversity of Gallibacterium anatis isolates from different chicken flocks |
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Journal articleJournal article |
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Forfatter:
Bojesen, A.M.
Technical University of Denmark
Forfatter:
Torpdahl, Mia
Technical University of Denmark, National Veterinary Institute
Forfatter:
Christensen, H.
Technical University of Denmark
Forfatter:
Olsen, J.E.
Technical University of Denmark
Forfatter:
Bisgaard, M.
Technical University of Denmark
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| Uddrag:
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Amplified fragment length polymorphisms (AFLPs) were used to characterize the genotypic diversity of a total of 114 Gallibacterium anatis isolates originating from a reference collection representing 15 biovars from four countries and isolates obtained from tracheal and cloacal swab samples of chickens from an organic, egg-producing flock and a layer parent flock. A subset of strains was also characterized by pulsed-field gel electrophoresis and biotyping. The organic flock isolates were characterized by more than 94% genetic similarity, indicating that only a single clone was apparent in the flock. The layer parent flock isolates were grouped into two subclusters, each with similarity above 90%. One subcluster contained only tracheal isolates, while the other primarily included cloacal isolates. In conclusion, we show that AFLP analysis enables fingerprinting of G. anatis, which seems to have a clonal. population structure within natural populations. There was further evidence of clonal lineages, which may have adapted to different sites within the same animal. |
| Publiceret:
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in journal: Journal of Clinical Microbiology (ISSN: 0095-1137) (DOI: http://dx.doi.org/10.1128/JCM.41.6.2737-2740.2003), vol: 41, issue: 6, pages: 2737-2740, 2003 |
| DOI:
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