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Titel: Isolation, structural elucidation and in vitro activity of 2-acetyl-2-decarboxamido-oxytetracycline against environmental relevant bacteria, including tetracycline-resistant bacteria
Type: Journal articleJournal article
Person(er):
Forfatter:  Lykkeberg, Anne Kruse
Technical University of Denmark

Forfatter:  Sengeløv, Gitte
Technical University of Denmark, National Veterinary Institute

Forfatter:  Cornett, Claus
Technical University of Denmark

Forfatter:  Tjørnelund, Jens
Technical University of Denmark

Forfatter:  Hansen, Steen Honoré
Technical University of Denmark

Forfatter:  Halling-Sørensen, Bent
Technical University of Denmark
Uddrag: 2-Acetyl-2-decarboxamido-oxytetracycline (ADOTC) is a major impurity of oxytetracycline (OTC) produced as a side product during fermentation. ADOTC was isolated from OTC and other impurities using preparative HPLC. The preparative column was an Xterra MS. C-18 chromatographic column (100 mm x 19 turn W., 5 mum), and the mobile phase contained methanol-water (27:73 (v/v)) with 0.08 M formic acid added. The flow rate was 9.0 ml/min. It was possible to isolate few milligram ADOTC in a day. The compound was unambiguously identified using NMR and MS-MS. The anti-microbial activity against activated sludge bacteria was deter-mined giving a potency of only 3% of that of OTC. With tetracycline-resistant bacteria, no anti-microbial activity was observed, indicating a mode of action similar to that of OTC.
Publiceret: in journal: Journal of Pharmaceutical and Biomedical Analysis (ISSN: 0731-7085) (DOI: http://dx.doi.org/10.1016/S0731-7085(03)00629-0), vol: 34, issue: 3, pages: 559-567, 2004
DOI:
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