Mette Theilgaard MRSA

New methods to identify MRSA in pigs

Tuesday 05 Aug 14

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Frank Møller Aarestrup
Professor, research manager
National Food Institute
+45 35 88 62 81

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Yvonne Agersø
Senior Reseacher
National Food Institute
+45 35 88 62 73

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Henrik Hasman
Senior Reseacher
National Food Institute
+45 35 88 63 47
It is important to keep the number of MRSA infections at a low level. In a PhD project at the National Food Institute, Technical University of Denmark, the latest technologies within whole genome sequencing were exploited to develop new methods to identify genes which are important for the survival of MRSA in pigs.

Traditionally, MRSA has been associated with severe infections which occur in hospitals and result in prolonged diseases and increased mortality. However, in recent years, MRSA has spread to the rest of the community, e.g. MRSA CC398 is found in pig production.

MRSA is resistant to the antibiotics we normally use for treatment of Staphylococcus infections.

In her PhD project at the National Food Institute, PhD student Mette Theilgaard exploited the latest technologies within whole genome sequencing and studied the entire DNA of MRSA CC398.

These studies have resulted in new methods, high-throughput approaches, which can identify genes important for the survival of MRSA in pigs. High-throughput approaches can identify those genes in the total gene pool of the bacteria which are essential, or the presence of which is advantageous, for the bacteria under some given circumstances.

  

Transfer of disease from animals to humans

The fact that MRSA can spread from animals to humans, where they may result in infections, has caused great concern in recent years. LA-MRSA ST398 is a new type and has turned out to be particularly successful in colonisation of pigs, from where it may transmit to humans.

Therefore, several monitoring studies focus on locating the origin of this MRSA type and its potential. Several central questions still remain unanswered, which makes it difficult to control the spread of this MRSA type. LA-MRSA ST398 is zoonotic, i.e. it can be transferred directly from animals to humans and cause disease. Thus, it is not sufficient to eradicate the bacteria from humans.

LA-MRSA ST398 has proven to be particularly successful in colonisation of pigs. By studying which genes are essential for the bacteria in pigs it may be possible for researchers to identify the factors important for the bacterium to colonise on pigs. We still don’t know which specific genetic factors in this MRSA type facilitate the spread from animals to humans. 

Read more

Read Mette Theilgaard’s PhD thesis:: Characterization of methicillin-resistant Staphylococcus aureus Sequence Type 398 (pdf). 


Facts about MRSA

MRSA stands for methichillin-resistant Staphylococcus aureus.

When bacteria are exposed to antibiotics they protect themselves by developing resistance. They do this by changing their genes, either by mutating or by transferring resistance genes among themselves. It is, therefore, important not to overconsume antibiotics but only to use the necessary amount.

Staphylococci are bacteria which can be found in humans, animals and our surroundings.
Staphylococcus aureus is part of the normal nasal and skin flora of approximately 50 % of the population. Staphylococcus aureus may cause various infections, ranging from superficial wounds and abscesses to severe infections such as bone inflammation and infection of the heart valve. In hospitals, Staphylococcus aureus is the most frequent cause of infections following surgery.

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