Researchers have found a way to engineer the lipid composition of cell membranes. The method described in Science makes it easier to investigate how cells are regulated by their membrane composition. This could lead to optimized cell factories and better understanding of the role of lipids in metabolic diseases, such as type 2 diabetes.
Today, much of the biology underlying cells’ compositions of lipids is a black box to scientists. Even though lipids are a major group of biomolecules, they are difficult to study because their synthesis is regulated by complicated metabolism.
Now, researchers from the Department of Energy’s Joint BioEnergy Institute (JBEI), Berkeley Lab, and The Novo Nordisk Foundation Center for Biosustainability at Technical University of Denmark (DTU Biosustain) have found a way to engineer the fatty membranes of cells. The researchers boosted the cells’ ability to produce high amounts of fluid fats, so-called unsaturated lipids. This increased the membrane respiration as well as the cell’s growth rate.
This knowledge could be an advantage when engineering the cellular workhorses E. coli or baker’s yeast to produce biofuels, biochemicals or biopharmaceuticals. Therefore, this method could become very important in industries working with cell factories.
The research has now been published in Science.
Discovering the causes for type 2 diabetes
The researchers showed how to manipulate the lipid composition of E. coli and baker’s yeast. The next step is to show that the method works for mammalian cells as well.
"This basic knowledge is very important in order to develop new treatments for diseases that we basically don’t understand the mechanisms behind"
Professor at JBEI and Scientific Director at DTU Biosustain Jay Keasling
“The applications for future work aren't industrial, but rather related to health. Several diseases, such as type 2 diabetes, lead to more rigid membranes and also feature impaired mitochondria function,” says first-author, Postdoc Itay Budin from JBEI.
Mitochondria are the cell’s power plants. Cells with impaired mitochondrial function work slowly compared to normal cells. Making the cells’ membrane more fluid could normalize mitochondrial function.
“We are excited about this work – it shows that synthetic biology and metabolic engineering can be applied to gain basic insights in fundamental biological processes. This basic knowledge is very important in order to develop new treatments for diseases that we basically don’t understand the mechanisms behind,” says co-author, Professor at JBEI and Scientific Director at DTU Biosustain Jay Keasling.
Resources:
Budin et al., Viscous control of cellular respiration by membrane lipid composition, Science 2018.