Postdoc in Cell-based Bioprocess and Computational Modeling

Friday 05 Jul 19

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Quantitative Modelling of Cell Metabolism (QMCM) group headed by Professor Lars Keld Nielsen at DTU, Copenhagen, is looking for Chemical Engineer with experience mammalian cells bioprocesses and metabolic modeling, for a postdoc position.

Group specializes in creating complex composite mechanistic / data-driven models of cellular metabolism. Such accurate and interpretable models can be used to analyze response to drugs, search for a new pharmaceutical targets or explore basic biology.

Quantitative Modelling of Cell Metabolism (QMCM) is a new section at the Novo Nordisk Foundation Center for Biosustainability (CfB), headed by Professor Lars Keld Nielsen and supported by a seven year Novo Nordisk Foundation Laureate Research Grant. In QMCM, we focus on developing mathematical models to explore and explain the molecular basis for homeostasis – the self-regulating processes evolved to maintain metabolic equilibrium in organisms.

This is an opportunity for a Chemical Engineer with expertise in mammalian cells bioprocesses to join an interdisciplinary team working on biological network modelling, computer science and algorithmics, statistical computation and bioinformatics, to solve the fundamental challenge of formulating and fitting very large non-linear models. 

Responsibilities and tasks 
The successful candidate will be primarily responsible for providing high-throughput quantitative multi-omic data to biological researchers and performing primary metabolic computational modeling analyses. The candidate is expected to have extensive experience in mammalian cell culture laboratory and will closely collaborate with other groups, both within the center and externally. Key responsibilities will include: 

  • Mammalian cell-based bioprocess optimization and automatation in different strategies (batch, fed-batch, continuous, perfusion)
  • Sample preparation, data acquirement and processing
  • Metabolic flux analysis in both steady-state and dynamics (FBA, D-FBA)
  • Multi-omic data analysis (metabolomics, transcriptomics, and proteomics)
  • 13C metabolic data generation and analysis to validate fluxes in metabolic models.
  • Setting up protocols and training other personnel to the highest standards
  • Coordinating the work undertaken by QMCM with CfB and others
  • Preparation of reports and manuscripts for publication
Qualifications
Candidates should have a PhD degree in biotechnology or a similar degree. The ideal candidates will have: 
  • Significant experience in cell culture and metabolomics analysis
  • Proven experience in chromatography method, mass spectrometry & high throughput analysis
  • Experience with sample preparation for metabolomics
  • Experience with different bioreactors platform at different scales: ambr, DASGIP, Biostat
  • Knowledge of analytical equipment for cell culture/fermentation: HPLC, GC-MS, LC-MS, ELISA, etc.
  • Experimental design using mammalian cells, although other organisms as E. coli and yeast are valuable.
  • Experience in computational metabolic modeling in steady-state (FBA) and Dynamic-FBA
  • Metabolic data integration into computational modeling of cell metabolism
  • Programming skills in Matlab
  • Capable of working on their own initiative when appropriate
  • Proven ability to work creatively in a team environment (including multi-disciplinary environment)
  • Excellent interpersonal, networking and communication skills
  • Strong oral and written skills in English
  • Flexibility, enthusiasm, responsibility, team spirit and excellent collaboration skills.
  • Computer proficiency (MS office) and Experience in reporting in lab notebooks and/or LIMS systems.
We offer
DTU is a leading technical university globally recognized for the excellence of its research, education, innovation and scientific advice. We offer a rewarding and challenging job in an international environment. We strive for academic excellence in an environment characterized by collegial respect and academic freedom tempered by responsibility. 

Salary and terms of employment
The appointment will be based on the collective agreement with the Danish Confederation of Professional Associations. The allowance will be agreed upon with the relevant union. The period of employment is 4 years.

You can read more about
career paths at DTU here

Further information
 
Further information may be obtained from Scientific Director Lars Keld Nielsen, lakeni@biosustain.dtu.dk.   

You can read more about The Novo Nordisk Foundation Center for Biosustainability on www.biosustain.dtu.dk/english.  

Application procedure
Please submit your online application no later than 19 July 2019 (local time). Applications must be submitted as one PDF file containing all materials to be given consideration. To apply, please open the link "Apply online", fill out the online application form, and attach all your materials in English in one PDF file. The file must include: 
  • Application (cover letter)
  • CV
  • Diploma (MSc/PhD)
  • List of publications
Applications and enclosures received after the deadline will not be considered.

All interested candidates irrespective of age, gender, disability, race, religion or ethnic background are encouraged to apply.

DTU Biosustain is an international research center of excellence developing next generation cell factories and bioprocesses for sustainable production of high-value chemical compounds as well as protein-based products. The center uses advanced metabolic engineering techniques and computational biology ensuring efficient and cost-effective design and construction processes.

The center’s activities are a balanced mix of basic and translational research, complemented by an emphasis on business development to facilitate commercialization of new cell factories and associated technologies. DTU Biosustain offers state-of-the-art research facilities and assembles world leaders in the field thus offering a unique platform providing excellent talent development and career opportunities.

DTU is a technical university providing internationally leading research, education, innovation and scientific advice. Our staff of 6,000 advance science and technology to create innovative solutions that meet the demands of society, and our 11,200 students are being educated to address the technological challenges of the future. DTU is an independent university collaborating globally with business, industry, government and public agencies.