Postdoc positions in Engineering Secondary Metabolite Biosynthesis in Actinomycetes

onsdag 18 aug 21

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Frist 30. september 2021
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We are seeking highly skilled and motivated postdoctoral scientists to join our international team at the Novo Nordisk Foundation Center for Biosustainability (DTU Biosustain), Technical University of Denmark (DTU), and become part of the Natural Products Genome Mining group headed by Prof. Tilmann Weber.

Within the Novo Nordisk Foundation funded Challenge Grant project “Integration of Informatics and Metabolic Engineering for the discovery of Novel Antibiotics (IIMENA)” you will actively contribute to develop and implement technologies in the field of genome mining and genetic and metabolic engineering of actinomycetes to identify and produce novel secondary metabolites as potential future antibiotics.

IIMENA is an international excellence project involving partner institutions from Spain and South Korea.

Responsibilities and qualifications
Within the IIMENA project, we are using different screening methods (HTS, co-ALE) to identify producers of novel bioactive molecules, including rare actinomycete taxa. We obtain high-quality genome sequences of interesting candidate strains and integrate these data in the screening, prioritization and dereplication process.

Are you our new co-worker who helps us to strengthen the actinomycete molecular biology / genetic engineering approaches that follow downstream within the project?

Your tasks will be centered around

  • Genome Mining studies on selected strains in collaboration with our computational biologists and collaboration partners
  • Generating transcriptome and proteome profiles of wild type secondary metabolite producing actinomycetes and chassis strains and analysing these datasets
  • Identification and characterization of biosynthetic gene clusters (BGCs) of target compounds by genetic engineering of the producer strains or cloning & heterologous expression
  • Development and implementation of methods to activate silent BGCs
  • Optimization of our set of tools to engineer rare actinomycetes, for example by improving our CRISPR toolkit, developing/implementing new cloning tools

As a formal qualification, you must hold a PhD degree (or equivalent).

You furthermore have

  • documented experience in working with filamentous actinomycetes.
  • excellent laboratory skills, practical experience of state-of-the-art molecular microbiology methods and tools. Knowledge and previous experience related to natural products biosynthesis is advantageous.
  • Good knowledge of relevant genome mining approaches and general bioinformatics methods that enables you to identify biosynthetic gene clusters, regulators or other interesting enzymes in large sequencing datasets; basic programming/large-scale data analysis skills are advantageous.
  • ambition, strong work ethics and attention to detail
  • excellent English communication and writing skills
  • excellent team-playing skills, but also the ability to work independently towards applied research goals in a multi-disciplinary international team of scientists.

We offer
DTU is a leading technical university globally recognized for the excellence of its research, education, innovation and scientific advice. We offer a rewarding and challenging job in an international environment. We strive for academic excellence in an environment characterized by collegial respect and academic freedom tempered by responsibility.

The Natural Products Genome Mining group at DTU Biosustain is a highly dynamic team of technicians, research assistants, PhD students, postdocs, researchers, and Professor Tilmann Weber. We work closely together to achieve our ambitious goals in creative and interesting ways by using, improving, and developing technologies such as nanopore sequencing, BGC cloning, high throughput screening, CRISPR base editing, and the antiSMASH BGC prediction platform.

Salary and terms of employment
The appointment will be based on the collective agreement with the Danish Confederation of Professional Associations. The allowance will be agreed upon with the relevant union.

The positions are limited to 31.8.2023. The positions are available immediately.

You can read more about career paths at DTU here.

Further information
Further information may be obtained from Prof. Tilmann Weber, preferably by email (tiwe@biosustain.dtu.dk). Please do not send applications to this e-mail address, instead apply online as described below.

You can read more about DTU Biosustain at www.biosustain.dtu.dk

If you are applying from abroad, you may find useful information on working in Denmark and at DTU at DTU – Moving to Denmark.

Application procedure
Your complete online application must be submitted no later than 30 September 2021 (Danish time).

Applications must be submitted as one PDF file containing all materials to be given consideration. To apply, please open the link "Apply online", fill out the online application form, and attach all your materials in English in one PDF file. The file must include:

  • Application (cover letter)
  • CV
  • Academic Diplomas (MSc/PhD)
  • List of publications

All interested candidates irrespective of age, gender, disability, race, religion or ethnic background are encouraged to apply.

The Novo Nordisk Foundation Center for Biosustainability (DTU Biosustain)
Recent progress in our ability to read and write genomic code, combined with advances in automation, analytics and data science, has fundamentally changed the scope and ambition of harnessing the potential of biological systems. Big data approaches and analysis of biological systems are key research instruments at the Center. DTU Biosustain utilizes these advances for microbial cell factory design to foster sustainable lifestyles in relation to three application areas: Sustainable Chemicals, Natural Products, and Microbial Foods.

Technology for people
DTU develops technology for people. With our international elite research and study programmes, we are helping to create a better world and to solve the global challenges formulated in the UN’s 17 Sustainable Development Goals. Hans Christian Ørsted founded DTU in 1829 with a clear vision to develop and create value using science and engineering to benefit society. That vision lives on today. DTU has 12,900 students and 6,000 employees. We work in an international atmosphere and have an inclusive, evolving, and informal working environment. DTU has campuses in all parts of Denmark and in Greenland, and we collaborate with the best universities around the world.