Research Assistant in CHO Cell Culture & Molecular Biology

torsdag 08 okt 20

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Frist 22. oktober 2020
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The CHO cell line engineering & Design (CLED) Section at the Novo Nordisk Foundation Center for Biosustainability is headed by Professor Gyun Min Lee. The CLED section addresses the need of rapidly obtaining high yields and high quality of biopharmaceuticals produced in optimised CHO cells through genome-scale-based methodologies. The section's main goal is to develop state-of-the-art genome editing technologies, omics data generation and high-throughput screening methods to improve CHO cell engineering efforts and facilitate identification of factors that control growth, protein production and processing.

We are looking for a research assistant skilled in cell culture and molecular biology, to support several of our research projects including secretory pathway engineering to optimize therapeutic protein production and rapid cell line engineering for production of new therapeutic proteins.

Responsibilities and tasks
As a member of a multi-disciplinary team, you will contribute your skills towards several research projects. Your key focus will be on cloning of expression plasmids for screening combinations of therapeutic product genes and effector genes, and additionally identify strategies for optimal mAb production. Overall, you will enable the team by providing the necessary support in both molecular biology and cell culture.

Examples of challenges you will address include:

  • Assist researchers with their design and development of experiments
  • Independently clone mammalian expression vectors for targeted integration in CHO cells.
  • Transfect expression vectors and generate stable clones
  • Run CHO cell batch cultures, and thoroughly characterize clones and phenotypes.

Qualifications
Candidates should have a master’s degree in biotechnology or equivalent.

A successful candidate will have:

  • Experience with cultivating CHO cells
  • Experience in cloning expression plasmids for mammalian cells (it is a plus if you are familiar with USER cloning)
  • Experience with different transfection methods
  • Experience with monoclonal antibody production
  • Experience with titer assays (e.g using plate reader, Octet or ELISA) and western blotting
  • A good understanding biopharmaceutical research and development, from drug discovery to production

In addition, we expect you to be interested in collaboration and take personal responsibility for your work. CFB is a multicultural environment with almost 50 nationalities. In order to contribute effectively in this environment, candidates are expected to be skilled communicators both in spoken and written English.

We offer
DTU is a leading technical university globally recognized for the excellence of its research, education, innovation and scientific advice. We offer a rewarding and challenging job in an international environment. We strive for academic excellence in an environment characterized by collegial respect and academic freedom tempered by responsibility.

Salary and terms of employment
The appointment will be based on the collective agreement with the Confederation of Professional Associations. The allowance will be agreed upon with the relevant union.

The period of employment stops 31.12.2020.

You can read more about career paths at DTU here.

Further information
Further information may be obtained from Lise Marie Grav, tel.: +45 8175 8092.

You can read more about DTU Biosustain department at https://www.biosustain.dtu.dk

Application procedure
Please submit your online application no later than 22 October 2020 (23:59 local time).

Applications must be submitted as one PDF file containing all materials to be given consideration. To apply, please open the link "Apply online", fill out the online application form, and attach all your materials in English in one PDF file. The file must include:

  • Application (cover letter)
  • CV
  • Diploma (MSc)

Applications and enclosures received after the deadline will not be considered.

All interested candidates irrespective of age, gender, disability, race, religion or ethnic background are encouraged to apply.

The Novo Nordisk Foundation Center for Biosustainability (DTU Biosustain)
Recent progress in our ability to read and write genomic code, combined with advances in automation, analytics and data science, has fundamentally changed the scope and ambition of harnessing the potential of biological systems. Big data approaches and analysis of biological systems are key research instruments at the Center. DTU Biosustain utilizes these advances for microbial cell factory design to foster sustainable lifestyles in relation to three application areas: Sustainable Chemicals, Natural Products, and Microbial Foods.

Technology for people
DTU develops technology for people. With our international elite research and study programmes, we are helping to create a better world and to solve the global challenges formulated in the UN’s 17 Sustainable Development Goals. Hans Christian Ørsted founded DTU in 1829 with a clear vision to develop and create value using science and engineering to benefit society. That vision lives on today. DTU has 12,000 students and 6,000 employees. We work in an international atmosphere and have an inclusive, evolving, and informal working environment. Our main campus is in Kgs. Lyngby north of Copenhagen and we have campuses in Roskilde and Ballerup and in Sisimiut in Greenland