Morten Nørholm

Morten Nørholm

Senior Researcher

DTU BIOSUSTAIN
Novo Nordisk Foundation Center for Biosustainability

CFB-PhD Komiteen

Danmarks Tekniske Universitet

Kemitorvet

Bygning 220, rum 313F

2800 Kgs. Lyngby

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2019
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Meta synthetic biology: controlling the evolution of engineered living systems

Nørholm, Morten H. H.
in: Microbial Biotechnology, vol: 12, issue: 1, pages: 35-37

Type: Journal article (Peer reviewed)

Status: Published     |    År: 2019     |    DOI: https://doi.org/10.1111/1751-7915.13350

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Mutations in the Global Transcription Factor CRP/CAP: Insights from Experimental Evolution and Deep Sequencing

Frendorf, Pernille Ott ; Lauritsen, Ida ; Sekowska, Agnieszka ; Danchin, Antoine ; Nørholm, Morten H.H.
in: Computational and Structural Biotechnology Journal, vol: 17, pages: 730-736

Type: Journal article (Peer reviewed)

Status: Published     |    År: 2019     |    DOI: https://doi.org/10.1016/j.csbj.2019.05.009

2018
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A synbio approach for selection of highly expressed gene variants in Gram-positive bacteria

Ferro, Roberto ; Rennig, Maja ; Hernández Rollán, Cristina ; Daley, Daniel O. ; Nørholm, Morten H H
in: Microbial Cell Factories, vol: 17, issue: 1

Type: Journal article (Peer reviewed)

Status: Published     |    År: 2018     |    DOI: https://doi.org/10.1186/s12934-018-0886-y

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BacHBerry:: BACterial Hosts for production of Bioactive phenolics from bERRY fruits

Dudnik, Alexey ; Almeida, A. Filipa ; Andrade, Ricardo ; Avila, Barbara ; Bañados, Pilar ; Barbay, Diane ; Bassard, Jean Etienne ; Benkoulouche, Mounir ; Bott, Michael ; Braga, Adelaide ; Breitel, Dario ; Brennan, Rex ; Bulteau, Laurent ; Chanforan, Celine ; Costa, Inês ; Costa, Rafael S. ; Doostmohammadi, Mahdi ; Faria, Nuno ; Feng, Chengyong ; Fernandes, Armando ; Ferreira, Patricia ; Ferro, Roberto ; Foito, Alexandre ; Freitag, Sabine ; Garcia, Gonçalo ; Gaspar, Paula ; Godinho-Pereira, Joana ; Hamberger, Björn ; Hartmann, András ; Heider, Harald ; Jardim, Carolina ; Julien-Laferriere, Alice ; Kallscheuer, Nicolai ; Kerbe, Wolfgang ; Kuipers, Oscar P. ; Li, Shanshan ; Love, Nicola ; Marchetti-Spaccamela, Alberto ; Marienhagen, Jan ; Martin, Cathie ; Mary, Arnaud ; Mazurek, Vincent ; Meinhart, Camillo ; Sevillano, David Méndez ; Menezes, Regina ; Naesby, Michael ; Nørholm, Morten H.H. ; Okkels, Finn T. ; Oliveira, Joana ; Ottens, Marcel ; Parrot, Delphine ; Pei, Lei ; Rocha, Isabel ; Rosado-Ramos, Rita ; Rousseau, Caroline ; Sagot, Marie France ; Dos Santos, Claudia Nunes ; Schmidt, Markus ; Shelenga, Tatiana ; Shepherd, Louise ; Silva, Ana Rita ; da Silva, Marcelo Henriques ; Simon, Olivier ; Stahlhut, Steen Gustav ; Solopova, Ana ; Sorokin, Artem ; Stewart, Derek ; Stougie, Leen ; Su, Shang ; Thole, Vera ; Tikhonova, Olga ; Trick, Martin ; Vain, Philippe ; Veríssimo, André ; Vila-Santa, Ana ; Vinga, Susana ; Vogt, Michael ; Wang, Liangsheng ; Wang, Lijin ; Wei, Wei ; Youssef, Sandra ; Neves, Ana Rute ; Forster, Jochen
in: Phytochemistry Reviews, vol: 17, issue: 2, pages: 291-326

Type: Journal article (Peer reviewed)

Status: Published     |    År: 2018     |    DOI: https://doi.org/10.1007/s11101-017-9532-2

 

Bacterial Genome Editing Strategy for Control of Transcription and Protein Stability

Lauritsen, Ida ; Martinez, Virginia ; Ronda, Carlotta ; Nielsen, Alex Toftgaard ; Nørholm, Morten H. H.
part of: Synthetic Metabolic Pathways, pages: 27-37, 2018

Type: Book chapter (Peer reviewed)

Status: Published     |    År: 2018     |    DOI: https://doi.org/10.1007/978-1-4939-7295-1_3

 

LyGo: simple one-step cloning of LPMO expression vectors: from in silico design to lab-ready construct in one week!

Hernández Rollán, Cristina ; Falkenberg, Kristoffer Bach ; Rennig, Maja ; Nørholm, Morten
part of: Sustain Conference 2018, 2018, Technical University of Denmark (DTU), Lyngby, Denmark
Presented at:
Sustain conference 2018: Creating Technology for a Sustainable Society

Type: Conference abstract in proceedings (Peer reviewed)

Status: Published     |    År: 2018

 

Selection of Highly Expressed Gene Variants in Escherichia coli Using Translationally Coupled Antibiotic Selection Markers

Rennig, Maja ; Daley, Daniel O. ; Nørholm, Morten H. H.
part of: Synthetic Metabolic Pathways, pages: 259-268, 2018

Type: Book chapter (Peer reviewed)

Status: Published     |    År: 2018     |    DOI: https://doi.org/10.1007/978-1-4939-7295-1_16

 

Standardized Cloning and Curing of Plasmids

Lauritsen, Ida ; Kim, Se Hyeuk ; Porse, Andreas ; Nørholm, Morten H. H.
part of: Synthetic Biology. Methods in Molecular Biology, pages: 469-476, 2018, Humana Press, New York

Type: Book chapter (Peer reviewed)

Status: Published     |    År: 2018     |    DOI: https://doi.org/10.1007/978-1-4939-7795-6_28

 

TARSyn: Tunable Antibiotic Resistance Devices Enabling Bacterial Synthetic Evolution and Protein Production

Rennig, Maja ; Martinez, Virginia ; Mirzadeh, Kiavash ; Dunas, Finn ; Röjsäter, Belinda ; Daley, Daniel O ; Nørholm, Morten
in: A C S Synthetic Biology, vol: 7, issue: 2, pages: 432-442

Type: Journal article (Peer reviewed)

Status: Published     |    År: 2018     |    DOI: https://doi.org/10.1021/acssynbio.7b00200

2017
 

A Modular High-Throughput In Vivo Screening Platform Based on Chimeric Bacterial Receptors

Lehning, Christina Eva ; Heidelberger, Jan B ; Reinhard, John ; Nørholm, Morten H. H. ; Draheim, Roger R.
in: A C S Synthetic Biology, vol: 6, issue: 7, pages: 1315-1326

Type: Journal article (Peer reviewed)

Status: Published     |    År: 2017     |    DOI: https://doi.org/10.1021/acssynbio.6b00288

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An expression tag toolbox for microbial production of membrane bound plant cytochromes P450

Vazquez Albacete, Dario ; Cavaleiro, Mafalda ; Christensen, Ulla ; Seppala, Susanna ; Møller, Birger Lindberg ; Nørholm, Morten
in: Biotechnology and Bioengineering, vol: 114, issue: 4, pages: 751-760

Type: Journal article (Peer reviewed)

Status: Published     |    År: 2017     |    DOI: https://doi.org/10.1002/bit.26203

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A versatile one-step CRISPR-Cas9 based approach to plasmid-curing

Lauritsen, Ida ; Porse, Andreas ; Sommer, Morten Otto Alexander ; Nørholm, Morten
in: Microbial Cell Factories, vol: 16

Type: Journal article (Peer reviewed)

Status: Published     |    År: 2017     |    DOI: https://doi.org/10.1186/s12934-017-0748-z

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CRISPR/Cas9-based genome editing for simultaneous interference with gene expression and protein stability

Martinez, Virginia ; Lauritsen, Ida ; Hobel, Tonja ; Li, Songyuan ; Nielsen, Alex Toftgaard ; Nørholm, Morten
in: Nucleic Acids Research, vol: 45, issue: 20

Type: Journal article (Peer reviewed)

Status: Published     |    År: 2017     |    DOI: https://doi.org/10.1093/nar/gkx797

 

De-bugging and maximizing plant cytochrome P450 production in Escherichia coli with C-terminal GFP fusions

Christensen, Ulla ; Vazquez Albacete, Dario ; Søgaard, Karina Marie ; Hobel, Tonja ; Nielsen, Morten Thrane ; Harrison, Scott James ; Hansen, Anders Holmgaard ; Møller, Birger Lindberg ; Seppala, Susanna ; Nørholm, Morten
in: Applied Microbiology and Biotechnology, vol: 101, issue: 10, pages: 4103-4113

Type: Journal article (Peer reviewed)

Status: Published     |    År: 2017     |    DOI: https://doi.org/10.1007/s00253-016-8076-5

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Increasing the permeability of Escherichia coli using MAC13243

Muheim, Claudio ; Götzke, Hansjörg ; Eriksson, Anna U. ; Lindberg, Stina ; Lauritsen, Ida ; Nørholm, Morten ; Daley, Daniel O.
in: Scientific Reports, vol: 7

Type: Journal article (Peer reviewed)

Status: Published     |    År: 2017     |    DOI: https://doi.org/10.1038/s41598-017-17772-6

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Isolation and characterization of the E. coli membrane protein production strain Mutant56(DE3)

Baumgarten, Thomas ; Schlegel, Susan ; Wagner, Samuel ; Löw, Mirjam ; Eriksson, Jonas ; Bonde, Ida ; Herrgard, Markus ; Heipieper, Hermann J. ; Nørholm, Morten ; Slotboom, Dirk Jan ; de Gier, Jan-Willem
in: Scientific Reports, vol: 7

Type: Journal article (Peer reviewed)

Status: Published     |    År: 2017     |    DOI: https://doi.org/10.1038/srep45089

 

The CYP79A1 catalyzed conversion of tyrosine to (E)-p-hydroxyphenylacetaldoxime unravelled using an improved method for homology modeling

Vazquez Albacete, Dario ; Montefiori, Marco ; Kol, Stefan ; Motawia, Mohammed Saddik ; Møller, Birger Lindberg ; Olsen, Lars ; Nørholm, Morten H. H.
in: Phytochemistry, vol: 135, pages: 8-17

Type: Journal article (Peer reviewed)

Status: Published     |    År: 2017     |    DOI: https://doi.org/10.1016/j.phytochem.2016.11.013

2016
 

Codon optimizing for increased membrane protein production

Mirzadeh, K. ; Toddo, S. ; Nørholm, Morten ; Daley, Daniel O.
part of: Heterologous Expression of Membrane Proteins, pages: 53-61, 2016, Springer

Type: Book chapter (Peer reviewed)

Status: Published     |    År: 2016     |    DOI: https://doi.org/10.1007/978-1-4939-3637-3_4

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Generation of mutation hotspots in ageing bacterial colonies

Sekowska, Agnieszka ; Wendel, Sofie ; Nørholm, Morten ; Danchin, Antoine

Type: Working paper

Status: Published     |    År: 2016     |    DOI: https://doi.org/10.1101/041525

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Generation of mutation hotspots in ageing bacterial colonies

Sekowska, Agnieszka ; Wendel, Sofie ; Christian Fischer, Emil ; Nørholm, Morten H. H. ; Danchin, Antoine
in: Scientific Reports, vol: 6, issue: 1

Type: Journal article (Peer reviewed)

Status: Published     |    År: 2016     |    DOI: https://doi.org/10.1038/s41598-016-0005-4

 

SEVA Linkers: A Versatile and Automatable DNA Backbone Exchange Standard for Synthetic Biology

Kim, Se Hyeuk ; Cavaleiro, Mafalda ; Rennig, Maja ; Nørholm, Morten
in: A C S Synthetic Biology, vol: 5, issue: 10, pages: 1177–1181

Type: Journal article (Peer reviewed)

Status: Published     |    År: 2016     |    DOI: https://doi.org/10.1021/acssynbio.5b00257

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Side effects of extra tRNA supplied in a typical bacterial protein production scenario

Søgaard, Karina Marie ; Nørholm, Morten H. H.
in: Protein Science, vol: 25, issue: 11, pages: 2102–2108

Type: Journal article (Peer reviewed)

Status: Published     |    År: 2016     |    DOI: https://doi.org/10.1002/pro.3011

2015
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Stereo-specific synthesis of (13r)-manoyl oxide

Hamberger, Björn (Inventor) ; Pateraki, Eirini (Inventor) ; Møller, Birger Lindberg ; Nørholm, Morten ; Nielsen, Morten Thrane ; Ranberg, Johan Andersen (Inventor) ; Bohlman, Carl Jorg (Inventor) ; Zerbe, Philip (Inventor)
Patent no.: WO2015091943, Date: 2015-06-25

Type: Patent

Status: Published     |    År: 2015

 

Accurate Dna Assembly And Direct Genome Integration With Optimized Uracil Excision Cloning To Facilitate Engineering Of Escherichia Coli As A Cell Factory

Cavaleiro, Mafalda ; Kim, Se Hyeuk ; Nørholm, Morten
part of: The Danish Microbiological Society Annual Congress 2015, pages: 77-77, 2015, Copenhagen
Presented at:
The Danish Microbiological Society Annual Congress 2015

Type: Conference abstract in proceedings (Peer reviewed)

Status: Published     |    År: 2015

 

Accurate DNA assembly and genome engineering with optimized uracil excision cloning

Cavaleiro, Mafalda ; Kim, Se Hyeuk ; Seppala, Susanna ; Nielsen, Morten Thrane ; Nørholm, Morten H. H.
in: A C S Synthetic Biology, vol: 4, issue: 9, pages: 1042-1046

Type: Journal article (Peer reviewed)

Status: E-pub ahead of print     |    År: 2015     |    DOI: https://doi.org/10.1021/acssynbio.5b00113

 

An Antibiotic Selection System For Protein Overproducing Bacteria

Rennig, Maja ; Nørholm, Morten
part of: The Danish Microbiological Society Annual Congress 2015, pages: 76-77, 2015, Copenhagen
Presented at:
The Danish Microbiological Society Annual Congress 2015

Type: Conference abstract in proceedings (Peer reviewed)

Status: Published     |    År: 2015

 

Assembly of Highly Standardized Gene Fragments for High-Level Production of Porphyrins in E. coli

Nielsen, Morten Thrane ; Madsen, Karina Marie ; Seppala, Susanna ; Christensen, Ulla ; Riisberg, Lone ; Harrison, Scott James ; Møller, Birger Lindberg ; Nørholm, Morten
in: A C S Synthetic Biology, vol: 4, issue: 3, pages: 274-282

Type: Journal article (Peer reviewed)

Status: Published     |    År: 2015     |    DOI: https://doi.org/10.1021/sb500055u

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Conditional protein depletion using small degradation tags and CRISPR/Cas9 systems

Martinez, Virginia ; Lauritsen, Ida ; Wolff, Tonja ; Nørholm, Morten H.H.
part of: Book of Abstracts. DTU's Sustain Conference 2015, 2015, Technical University of Denmark (DTU), Lyngby
Presented at:
DTU Sustain Conference 2015

Type: Conference abstract in proceedings (Peer reviewed)

Status: Published     |    År: 2015

 

Enhanced Protein Production in Escherichia coli by Optimization of Cloning Scars at the Vector-Coding Sequence Junction

Mirzadeh, Kiavash ; Martinez, Virginia ; Toddo, Stephen ; Guntur, Suchithra ; Herrgard, Markus ; Elofsson, Arne ; Nørholm, Morten ; Daley, Daniel O.
in: A C S Synthetic Biology, vol: 4, issue: 9, pages: 959-965

Type: Journal article (Peer reviewed)

Status: E-pub ahead of print     |    År: 2015     |    DOI: https://doi.org/10.1021/acssynbio.5b00033

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Expanding the N-terminal tag toolbox for functional expression of Cytochrome P450s in cell factories

Vazquez Albacete, Dario ; Cavaleiro, Mafalda ; Christensen, Ulla ; Seppälä, Susanna ; Nørholm, Morten H.H.
part of: Book of Abstracts. DTU's Sustain Conference 2015, 2015, Technical University of Denmark (DTU), Lyngby
Presented at:
DTU Sustain Conference 2015

Type: Conference abstract in proceedings (Peer reviewed)

Status: Published     |    År: 2015

 

Exploring Marine Environments To Unravel Tolerance Mechanisms To Relevant Compounds

Machado, Henrique ; Cavaleiro, Mafalda ; Nørholm, Morten ; Gram, Lone
part of: The Danish Microbiological Society Annual Congress 2015, pages: 76-76, 2015, Copenhagen
Presented at:
The Danish Microbiological Society Annual Congress 2015

Type: Conference abstract in proceedings (Peer reviewed)

Status: Published     |    År: 2015

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Superlinkers – A versatile and automatable DNA backbone exchange standard for synthetic biology

Kim, Se Hyeuk ; Cavaleiro, Mafalda ; Riisberg, Lone ; Smith, Pernille ; Nørholm, Morten
Presented at:
Copenhagen Bioscience Conference 2015

Type: Conference abstract for conference (Peer reviewed)

Status: Published     |    År: 2015

 

Uracil Excision for Assembly of Complex Pathways

Cavaleiro, Mafalda ; Nielsen, Morten Thrane ; Kim, Se Hyeuk ; Seppala, Susanna ; Nørholm, Morten
part of: Springer Protocols Handbooks, 2015, Humana Press

Type: Book chapter (Peer reviewed)

Status: Published     |    År: 2015     |    DOI: https://doi.org/10.1007/8623_2015_133

2014
 

‘Forcing the issue’ - Aromatic tuning facilitates stimulus-independent modulation of a two-component signaling circuit

Nørholm, Morten ; von Heijne, Gunnar ; Draheim, Roger R.
in: A C S Synthetic Biology, vol: 4, issue: 4, pages: 474-481

Type: Journal article (Peer reviewed)

Status: Published     |    År: 2014     |    DOI: https://doi.org/10.1021/sb500261t

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Microbial Synthesis of the Forskolin Precursor Manoyl Oxide in an Enantiomerically Pure Form

Nielsen, Morten Thrane ; Ranberg, Johan Andersen ; Christensen, Ulla ; Christensen, Hanne Bjerre ; Harrison, Scott James ; Olsen, Carl Erik ; Hamberger, Björn ; Møller, Birger Lindberg ; Nørholm, Morten
in: APPLIED AND ENVIRONMENTAL MICROBIOLOGY, vol: 80, issue: 23, pages: 7258–7265

Type: Journal article (Peer reviewed)

Status: Published     |    År: 2014     |    DOI: https://doi.org/10.1128/AEM.02301-14

 

Synthetic Biology Tools for the Membrane – Targeted Localisation and Elucidation of Protein Interactions

Wendel, Sofie ; Seppala, Susanna ; Nørholm, Morten
part of: Abstract Book - DTU Sustain Conference 2014, 2014, Technical University of Denmark (DTU), Kgs. Lyngby
Presented at:
DTU Sustain Conference 2014

Type: Conference abstract in proceedings (Peer reviewed)

Status: Published     |    År: 2014

2013
 

Improved production of membrane proteins in Escherichia coli by selective codon substitutions

Nørholm, Morten H.H. ; Toddo, Stephen ; Virkki, Minttu T.I. ; Light, Sara ; von Heijne, Gunnar ; Daley, Daniel O.
in: FEBS Letters, vol: 587, issue: 15, pages: 2352-2358

Type: Journal article (Peer reviewed)

Status: Published     |    År: 2013     |    DOI: https://doi.org/10.1016/j.febslet.2013.05.063

2012
 

Application of split-green fluorescent protein for topology mapping membrane proteins in Escherichia coli

Toddo, Stephen ; Soderstrom, Bill ; Palombo, Isolde ; von Heijne, Gunnar ; Nørholm, Morten ; Daley, Daniel O.
in: Protein Science, vol: 21, issue: 10, pages: 1571-1576

Type: Journal article (Peer reviewed)

Status: Published     |    År: 2012     |    DOI: https://doi.org/10.1002/pro.2131